Representatives for Iowa Citizens for Community Improvement and the Bakken Pipeline Resistance Coalition gathered Thursday morning outside the offices of the Iowa Utilities Board to submit the paperwork.
The board is scheduled later this year to decide whether to approve the pipeline, which would transport up to 570,000 barrels of crude oil daily from North Dakota through South Dakota and Iowa to a distribution center in Illinois.
The groups say the pipeline will affect the rights of landowners and hurt the environment.
The project was proposed by Dakota Access, LLC, a unit of Dallas-based Energy Transfer Partners. A Dakota Access spokeswoman was not immediately available for comment Thursday.